Computer network conditions that are dynamic and also complex make network operators often make mistakes in the configuration of a network device. However, these mistakes can be resolved by the emergence of a centralized programming approach which is now known as Software Defined Network (SDN). In addition, with the development of information technology, several services in computer networks also grabbed a lot of attention from the world, such as video streaming services. A video streaming service that is so massive is capable of having an impact on data traffic on computer networks. In SDN networks, controllers are a core component of this architecture. Currently, many different types of SDN controllers can be used, however, choosing a controller in an SDN network must be an important concern. This is related to the performance of the SDN controller which is a requirement for SDN network development. This research will analyze the performance of the video streaming service using the Real-time Transport Protocol (RTP) streaming protocol in testing two SDN controllers, namely Pox and Opendaylight. Based on the tests that have been carried out, it produces Quality of Service (QoS) values in the form of throughput, delay, and jitter. The Pox controller excels in the value of QoS delay and throughput. As for the jitter value, both Pox and Opendaylight controllers have good indexes.