In Vitro Antimicrobial Activity of Javanese Tamarind Leaves Infusion (Tamarindus indica Linn.) in Escherichia coli
AbstractEscherichia coli is the most common bacteria that cause diarrhea. Based on Kurniawatiâ€™s research, ethanol extract of tamarind leaves has an antimicrobial effect to Escherichia coli. This study aims to determine the antimicrobial effect using disk diffusion method, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of tamarind leaves infusion against Escherichia coli.Â This research was done using a real laboratory experimental design with tamarind leaves infusion and Escherichia coli microbes as the research objects. Zones of inhibition were observed using disk diffusion method by putting the tamarind leaves infusion disks on Mueller-Hinton agars with Ampicillin antibiotic for comparison, followed by observing the MIC using macro broth dilution methodÂ and MBC. The result of disk diffusion showed that tamarind leaves infusion had no zone of inhibition, whereas the mean value of the zones of inhibition of ampicillin is 17.2 mm. The MIC of tamarind leaves infusion is 62.5 mg/mL. Average growth of bacteria Â for the MBC of tamarind leaves infusion at 125 mg/mL and 62.5 mg/mL concentration are 13 CFU/mL and >300 CFU/mL. It can be concluded that tamarind leaves infusion has antimicrobial activity effect against E.coli and that effect is bacteriostatic.Â Keywords: Tamarindus indica Linn, Escherichia coli, MIC, MBC
Download data is not yet available.
How to Cite
Suryadi C, Rusmana D, Evacuasiany E. In Vitro Antimicrobial Activity of Javanese Tamarind Leaves Infusion (Tamarindus indica Linn.) in Escherichia coli. J. Med. Health [Internet]. 2015Feb.27 [cited 2023Mar.22];1(1). Available from: https://journal.maranatha.edu/index.php/jmh/article/view/498
Authors who publish with this journal agree to the following terms:
- Authors retain the copyright and grant the journal right of first publication with the work
simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the nonexclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.