Bitter Leaves Ethanol Extract (Vernonia amygdalina) Decreases Total Cholesterol Serum Level of Hypercholesterolemic Male Wistar Rats

  • Ivana C Hasan Universitas Kristen Maranatha Bandung
  • Hendra Subroto
  • Grace Puspasari

Abstract

Hypercholesterolemia is an important risk factors of atherosclerosis. Bitter leaf is believed to have potency in decreasing plasma lipid. The aim of this study is to study the effect of bitter leaf ethanol extract in decreasing total cholesterol serum levels on hypercholesterolemic male Wistar rats. This is a true experimental laboratory study with complete randomized design. Antidyslipidemia effect was tested with HFF induction and Propiltiourasil 0,01% towards 30 rats which were divided into 6 groups: groups I as normal control (standard diet), groups II, III, and IV were given bitter leaf ethanol extract of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW dose, group V as positive control group with Simvastatin, and group VI as negative with aquades. HFF  were given for 14 days then the study was continued for 14 days. The percentage data of the declining total cholesterol levels before and after treatments is analyzed with ANAVA, continued with LSD test. The result between group II, III, and IV with group VI shows a significant difference p<0,01. We concluded bitter leaf ethanol extract can decrease the level of total cholesterol serum of hypercholesterolmic Wistar rats.

References

Widyaningsih W. EEfek Ekstrak Etanol Rimpang Temugiring ( Curcuma heyneana Val .) terhadap Kadar Trigliserida. J Vet. 2011;1(1):55–65.

Goldberg A. Dyslipidemia [Internet]. 2015 [cited 2017 Dec 7]. Available from: https://www.msdmanuals.com/professional/endocrine-and-metabolic-disorders/lipid-disorders/dyslipidemia

Gibbons G. Atherosclerosis [Internet]. National Heart, Lung, and Blood Institute. 2013 [cited 2017 Dec 8]. Available from: https://www.nhlbi.nih.gov/health-topics/atherosclerosis

Cardiovaskular Diseases (CVDs) [Internet]. WHO. 2013 [cited 2017 Dec 8]. Available from: http://www.who.int/en/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds)

Penyakit Jantung Penyebab Kematian Tertinggi, Kemenkes Ingatkan Cerdik [Internet]. Kemenkes RI. 2017 [cited 2017 Dec 8]. Available from: http://www.depkes.go.id/article/view/17073100005/penyakit-jantung-penyebab-kematian-tertinggi-kemenkes-ingatkan-cerdik-.html

Erwinanto, Santoso A, Putranto JNE, Tedjasukmana P, Suryawan R, Rifqi S, et al. Pedoman tatalaksana dislipidemia. J Kardiol Indones [Internet]. 2013;34(4):245–70. Available from: http://jki.or.id

Oluwatosin A, Akintayo O, Achem J, Michael AF. Lipid-Lowering Effects of Methanolic Extract of Vernonia Amygdalinaleaves in Rats Fed nn High Cholesterol Diet. Vasc Heal Risk Manag. 2008;4:1.

Kalvari E. Efek Pemberian Rebusan Daun Afrika (Vernonia amygdalina) terhadap Kadar Kolesterol Total pada Tikus Jantan Galur Wistar yang Diinduksi Pakan Tinggi Lemak. Repos Univ Kristen Maranatha. 2015;

Arief M, Novriansyah R, Budianto IT, Bimo M, Harmaji. Potensi Bunga Karamuning (Melastoma malabathricum L.) terhadap Kadar Kolesterol Total dan Trigliserida pada Tikus Putih Jantan Hiperlipidemia yang diinduksi Propiltiourasil. J Prestasi. 2012;1(2):118–26.

Cannas A. Departement of Animal Science-Plants Poisonous to Livestock [Internet]. Cornel University. 2015 [cited 2018 Apr 14]. Available from: http://poisonousplants.ansci.cornell.edu/toxicagents/tannin.html

McRae MP. Vitamin C supplementation lowers serum low-density lipoprotein cholesterol and triglycerides: a meta-analysis of 13 randomized controlled trials. J Chiropr Med. 2008;7(2):48–58.

Sunarsih E sri, Pamuji S. Pengaruh Pemberian Vitamin C terhadap Kadar Kolesterol total Serum Tikus Jantan Hiperlipidemia Setelah Perlakuan Jus Lidah Buaya (Aloe vera Linn). J Pharm. 2007;5(2):120.

Atangwho IJ, Ebong PE, Enyong EU, Williams IO, Eteng MU, Egbung GE. Comparative Chemical Composition of Leaves of Some Antidiabetic Medicinal Plants: Azadirachta Indica, Vernonia Amygdalina and Gongronema Latifolium. African Journal of Biotechnology. 2009; 8(18): 4685-9.

Published
2018-08-31
Section
Articles